Peroxyfluor 1
CAS No. : 817170-66-4
| Size | Price | Stock |
|---|---|---|
| 5mg | $456 | In-stock |
| 10mg | $729 | In-stock |
| 25mg | $1530 | In-stock |
| 50mg | $2440 | In-stock |
| 100mg | $3900 | In-stock |
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| 500 mg | Get quote | |
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| Cat. No. : | HY-D1458 |
| M.Wt: | 552.23 |
| Formula: | C32H34B2O7 |
| Purity: | >98 % |
| Solubility: | DMSO : 14.29 mg/mL (ultrasonic;warming;adjust pH to 10 with 1M NaOH) |
Peroxyfluor 1 is a cell-permeable probe for H2O2. Peroxyfluor 1 represents a first-generation, green-fluorescent probe[1].
In Vitro:1. Solution preparation[1]
1.1 Preparation of stock solution
Solvent: DMSO
Concentration: 5 mM (optimized according to the experiment).
Storage: Store at -20°C or -80°C in dark after aliquoting. Avoid repeated freezing and thawing.
1.2 Preparation of working solution
Dilute to 5 µM with PBS or cell culture medium.
Note: The working solution should be prepared and used immediately. Keep it away from light.
2. Cell staining
2. Peroxyfluor 1 (5 μM) is incubated with live cell samples for 5-10 min.
2.2 Addition of H2O2 (10-100 μM) to cell samples for 15-30 min.
2.3 Fluorescence microscopy detection (Ex/Em = 450/530 nm).
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